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Elisa Serra-Casas, Paulo Manrique, Xavier C. Ding, Gabriel Carrasco-Escobar, Freddy Alava, Anthony Gave, Hugo Rodriguez, Juan Contreras-Mancilla, Angel Rosas-Aguirre, Niko Speybroeck, Iveth J. González, Anna Rosanas-Urgell, Dionicia Gamboa
Loop-mediated isothermal DNA amplification for asymptomatic malaria detection in challenging field settings: Technical performance and pilot implementation in the Peruvian Amazon
www.plosone.org
www.plosone.org
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(a)Tj
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(malaria)Tj
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1.0319 0 Td
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0.9921 0 Td
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[(\n)]TJ
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(centre\))Tj
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([)Tj
0.83 0.64 0.02 0 k
(28)Tj
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(a)Tj
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(\(occupational-related\).)Tj
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1.0318 0 Td
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(and)Tj
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(\(FSAT\))Tj
3.2031 0 Td
(strategy,)Tj
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3.5489 0 Td
[()]TJ
/F6 1 Tf
(.)Tj
/F13 1 Tf
[(\n)]TJ
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(.)Tj
1.3323 0 Td
(consecutive)Tj
4.8756 0 Td
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-34.6448 -1.2983 Td
(case)Tj
1.8595 0 Td
(detection)Tj
3.9401 0 Td
(screenings)Tj
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(in)Tj
1.0375 0 Td
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1.0262 0 Td
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1.2018 0 Td
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3.3959 0 Td
(increase)Tj
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(of)Tj
1.0148 0 Td
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1.0375 0 Td
(target)Tj
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(com-)Tj
-34.5144 -1.3039 Td
(munities)Tj
3.7303 0 Td
([)Tj
0.83 0.64 0.02 0 k
(29)Tj
0 g
(].)Tj
2.0806 0 Td
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1.1055 0 Td
(each)Tj
2.0296 0 Td
(screening,)Tj
4.2747 0 Td
(all)Tj
1.1508 0 Td
(community)Tj
4.8869 0 Td
(households)Tj
4.7338 0 Td
(are)Tj
1.4514 0 Td
(visited,)Tj
3.0444 0 Td
(all)Tj
1.1451 0 Td
(family)Tj
2.727 0 Td
(members)Tj
3.9458 0 Td
(are)Tj
-36.3059 -1.2983 Td
(diagnosed)Tj
4.2802 0 Td
(by)Tj
1.1792 0 Td
(light)Tj
2.0296 0 Td
(microscopy,)Tj
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(and)Tj
1.7291 0 Td
(all)Tj
1.1452 0 Td
(individuals)Tj
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(with)Tj
1.9956 0 Td
(a)Tj
0.6576 0 Td
(positive)Tj
3.3165 0 Td
(result)Tj
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(receive)Tj
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(antimalarial)Tj
-31.5323 -1.2983 Td
(treatment)Tj
4.1328 0 Td
(regardless)Tj
4.2066 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(presence)Tj
3.7133 0 Td
(of)Tj
1.0148 0 Td
(symptoms.)Tj
4.5864 0 Td
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4.0762 0 Td
(in)Tj
1.0318 0 Td
(spite)Tj
2.092 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(labour-intensive-)Tj
-29.8202 -1.3039 Td
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(of)Tj
1.0148 0 Td
(this)Tj
1.6781 0 Td
(strategy,)Tj
3.5489 0 Td
(it)Tj
0.7881 0 Td
(is)Tj
0.8447 0 Td
(still)Tj
1.6384 0 Td
(difficult)Tj
3.3562 0 Td
(to)Tj
1.0261 0 Td
(efficiently)Tj
4.1499 0 Td
(target)Tj
2.5058 0 Td
(and)Tj
1.7348 0 Td
(eliminate)Tj
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(the)Tj
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(remaining)Tj
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(reservoirs)Tj
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(mainly)Tj
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(due)Tj
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(to)Tj
1.0261 0 Td
(the)Tj
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(low)Tj
1.6554 0 Td
(sensitivity)Tj
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(being)Tj
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(paigns)Tj
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([)Tj
0.83 0.64 0.02 0 k
(23)Tj
0 g
(].)Tj
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1.8708 0 Td
(main)Tj
2.2847 0 Td
(objective)Tj
3.7814 0 Td
(was)Tj
1.7008 0 Td
(to)Tj
1.0261 0 Td
(evaluate)Tj
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(a)Tj
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(malaria)Tj
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(diagnostic)Tj
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(strategy)Tj
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(based)Tj
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(on)Tj
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(the)Tj
1.4684 0 Td
(use)Tj
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(of)Tj
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(LAMP)Tj
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(Pan/Pf)Tj
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(kits)Tj
1.6441 0 Td
(to)Tj
1.0261 0 Td
(detect)Tj
2.6136 0 Td
(asymptomatic)Tj
5.8563 0 Td
(infections,)Tj
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(in)Tj
1.0318 0 Td
(the)Tj
1.474 0 Td
(context)Tj
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(of)Tj
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(the)Tj
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(screening)Tj
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(campaigns)Tj
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(con-)Tj
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(ducted)Tj
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(by)Tj
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(field-mobile)Tj
5.0966 0 Td
(teams)Tj
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(\(brigadas\))Tj
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(Peruvian)Tj
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(we)Tj
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(analysed)Tj
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(of)Tj
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(LAMP)Tj
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(as)Tj
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(a)Tj
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(tool)Tj
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(for)Tj
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(detecting)Tj
3.8947 0 Td
(asymptomatic)Tj
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(infections)Tj
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(this)Tj
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(high-sensitive)Tj
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(molecular)Tj
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/F13 1 Tf
[()]TJ
/F6 1 Tf
(.)Tj
/F13 1 Tf
[(\n)]TJ
/F6 1 Tf
(.)Tj
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(real-time)Tj
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(reference)Tj
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(dard\))Tj
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(and)Tj
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[()]TJ
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[(\n)]TJ
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(microscopy\).)Tj
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(assessed)Tj
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(operational)Tj
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(introducing)Tj
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(this)Tj
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(procedures)Tj
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(teams,)Tj
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(including)Tj
4.0365 0 Td
(riverine)Tj
3.3505 0 Td
(screenings)Tj
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(and)Tj
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(an)Tj
1.2019 0 Td
(on-site)Tj
2.9763 0 Td
(evalua-)Tj
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1.8425 0 Td
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0.9467 0 Td
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(small)Tj
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(community)Tj
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(only)Tj
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Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 261.0141 cm
BT
/F2 1 Tf
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(Materials)Tj
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(and)Tj
1.989 0 Td
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(site)Tj
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Q
q
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BT
/F6 1 Tf
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1.7801 0 Td
(study)Tj
2.3924 0 Td
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(conducted)Tj
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(in)Tj
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(province,)Tj
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(Loreto)Tj
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(department,)Tj
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(Peruvian)Tj
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1.7858 0 Td
(annual)Tj
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(average)Tj
3.2258 0 Td
(temperature)Tj
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(is)Tj
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(around)Tj
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(27C,)Tj
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(with)Tj
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(an)Tj
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(average)Tj
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(annual)Tj
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(rainfall)Tj
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(4.000)Tj
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(and)Tj
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(a)Tj
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(humidity)Tj
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(above)Tj
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(80%)Tj
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([)Tj
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(30)Tj
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(transmission)Tj
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(in)Tj
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(and)Tj
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(July)Tj
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([)Tj
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(31)Tj
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(])Tj
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(and)Tj
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[()30()6()11(\r)10()6(\n)15()4(\n)8()]TJ
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[()4()16()8()12()12()13()-8()]TJ
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(main)Tj
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(vector)Tj
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([)Tj
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(32)Tj
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